Optimization of Polymer/Salt Aqueous Biphasic System (ABS) for Direct Recovery of Recombinant Bacillus Subtilis Protease

Mei Qi Gan, Phei Er Kee, Yin Hui Chow and Hui Suan Ng

Faculty of Applied Sciences, UCSI University, UCSI Heights, 56000 Cheras, Kuala Lumpur, Malaysia.

Centre for Research and Graduate Studies, University of Cyberjaya, Persiaran Bestari, 63000 Cyberjaya, Selangor, Malaysia.

School of Engineering, Taylor’s University, Lakeside Campus, No.1, Jalan Taylor’s 47500 Subang Jaya, Selangor, Malaysia.

*Corresponding author email: grraceng@cyberjaya.edu.my

ABSTRACT

Introduction: The growing market demand for protease in various industries has sparked the interest among researchers to develop a simple, cost-effective and high yield method for the downstream recovery of proteases.

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